Characterization of genes in the cellulose-synthesizing operon (acs operon) of Acetobacter xylinum: implications for cellulose crystallization
نویسندگان
چکیده
منابع مشابه
Synthesis of Cellulose by Acetobacter Xylinum
Appearance of cellulose microfibrils in the medium of a suspension of cells of Acetobacter xylinum in buffered glucose solution was preceded by a stage during which the cellulose in the medium was amorphous within the available resolution. The size of the vertical axis of the microfibrils of the bacterial cellulose was found on the basis of measurement of shadow length to be only about 16 A. In...
متن کاملA beta-glucosidase gene downstream of the cellulose synthase operon in cellulose-producing Acetobacter.
An open reading frame was found 214 bp downstream of the cellulose synthase operon of Acetobacter. The encoded amino acid sequence was found to be similar to some beta-glucosidases (G3ases). We detected G3ase activity in the culture medium and analysis of the N-terminal amino acid sequence showed that this gene encodes the enzyme. Therefore, it is possible that this region is a gene cluster for...
متن کاملAlternative Environmental Roles for Cellulose Produced by Acetobacter xylinum.
The cellulose-producing bacterium Acetobacter xylinum has been considered a strict aerobe, and it has been suggested that the function of cellulose is to hold cells in an aerobic environment. In this study, we showed that A. xylinum is capable of growing microaerophilically. Cellulose pellicles provided significant protection to A. xylinum cells from the killing effects of UV light. In experime...
متن کاملCellulose biogenesis: Polymerization and crystallization are coupled processes in Acetobacter xylinum.
Calcofluor White ST, stilbene derivative used commerically as an optical brightener for cellulose, increased the rate of glucose polymerization into cellulose by resting cells of the gram-negative bacterium Acetobacter xylinum. This bacterium normally produces a ribbon of cellulose that is a composite of crystalline microfibrils. In concentrations above 0.1 mM, Calcofluor disrupts the assembly ...
متن کاملThe synthesis of cellulose in cell-free extracts of Acetobacter xylinum.
In a previous publication (2) evidence was presented that the synthesis of chitin in Neurospora crassa occurs by way of a glycosyl transfer from uridine diphosphate-N-acetylglucosamine. The similarity of the structure of chitin and cellulose suggested that a like mechanism may be operative in the biosynthesis of cellulose. It has been shown by Hestrin and Schramm (3) that non-viable lyophilized...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1994
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.176.18.5735-5752.1994